Phospho stat3 flow cytometry
WebFlow cytometry quantification of phospho-STAT3, phospho-STAT5 and expression of Bcl-2/Bcl-XL after TQ treatment. Phosphorylation of STAT3 (A) and STAT5 (B) as well as the … WebFlow Cytometry CHARACTERIZATION This antibody is tested by direct flow cytometric analysis of human cells. WORKING DILUTIONS ... (green), using Phospho-Stat3 (Tyr705) (D3A7) XP Alexa Fluor 488 conjugate compared to nonspecific control antibody (red), and flow cytometric analysis of Jurkat cells, untreated (blue) or IFN-α treated (green), using ...
Phospho stat3 flow cytometry
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WebFeb 19, 2024 · Costunolide is a naturally occurring sesquiterpene lactone that demonstrates various therapeutic actions such as anti-oxidative, anti-inflammatory, and anti-cancer properties. Costunolide has recently emerged as a potential anti-cancer agent in various types of cancer, including colon, lung, and breast cancer. However, its mode of action in … WebMar 1, 2024 · The role of STAT3 in B cell responses to IFNα was studied using pharmacological inhibitors and PBMCs from STAT3-deficient individuals. Results: …
WebFlow Cytometry 0.25 µg/10 6 cells See below CyTOF-ready Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Immunocytochemistry 5-15 µg/mL See below Please Note: Optimal dilutions should be determined by each laboratory for each application. WebPhospho Targets Need a control for your phospho flow cytometry staining? Our Veri-Cells™ Phospho PBMCs can be used as a positive and negative control for staining members of …
WebA specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Intracellular Staining by Flow Cytometry View Larger Detection of Phospho-STAT3 (Y705) in IFN alpha-treated Daudi Human Cell Line by Flow Cytometry. WebApr 15, 2024 · a STAT3 activation in un-transfected and transfected with a cDNA coding for murine IL-11R murine myoblasts (C2C12) without cytokine (-) and after stimulation with HIL-11 (200 ng/ml), IL-11 (200 ng ...
WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-STAT3 (phospho S727) antibody [E121-31] (ab32143) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment – …
WebMar 14, 2013 · Additionally, STAT3 has a prolonged activation over other STATs and plays a critical role in the IL-21 signaling . pSTAT3 measurements resulted in a very good dynamic range for flow cytometry analysis allowing the generation of excellent dose response curves and a good IC 50 determinaion. Thus, phospho-STAT3 was selected as a biomarker for IL … state of michigan car rental discountWebWestern blot analysis for Stat3 (pY705). A431 cells (Human epithelial carcinoma; ATCC CRL-1555) were either left untreated (lane 1) or treated with 100 ng/ml EGF for 5 minutes at 37°C (lane 2). The top panel was probed with a mouse anti-Stat3 antibody measuring for total Stat3 (Cat. No. 610189) while the bottom panel was probed with a 1:500 ... state of michigan ccbhcWebSTAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the … state of michigan carpoolWebFlow cytometry can be used to study STAT phosphorylation on a per cell basis. Cells are fixed, permeablized, and stained with antibodies that specifically bind to the … state of michigan cbtWebJan 1, 2012 · Flow cytometry can be used to study STAT phosphorylation on a per cell basis. Cells are fixed, permeablized, and stained with antibodies that specifically bind to … state of michigan cci rulesWebPhosphoflow cytometry is increasingly being used as a tool for the discovery of biomarkers used in the treatment and monitoring of disease and therapy. The ability to measure numerous phospho-protein targets simultaneously at a single cell level accurately and rapidly provides significant advantages … state of michigan caregiversWebDec 26, 2024 · ROS production, apoptotic effect, and DNA contents of the cells were measured by flow cytometry. The two diarylheptanoids 1 and 3 and the AHC extract exhibited cytotoxic effects on MCF-7 cells in MTT assay, with IC50 values of 18.1, 46.9, 260.0 μg/mL, respectively. ... However, the levels of inflammatory markers including … state of michigan ccwis